The deoxycholate acts as a carrier that assists in the precipitation of protein. It is soluble in 100% (w/v) TCA, but not in 20% TCA. Thus, it precipitates out of solution upon dilution of the TCA + DOC mixture. ... Add ¼ volume of TCA + DOC to the protein fraction (in a 1.5 mL tube) to a final concentration of 20% (w/v).
TCA precipitation
//medicine.utah.edu/pathology/research/labs/dean.../TCA+DOC_precipitation.docx
How long oligopeptites can be precipitation by TCA ? - ResearchGate
//www.researchgate.net/.../How_long_oligopeptites_can_be_precipitation_by_TCA
3 answers
Proteins can be precipitated by TCA, can tripeptides, pentapeptides, ... by adding sodium deoxycholate or Sarkosyl as a carrier, as is the case with proteins. .... I want to measure the small peptide (2-3 kDa, 25 residues) concentration,
TCA protein precipitation
//research.fhcrc.org/content/dam/stripe/.../TCA%20protein%20precipitation.pdf
by K Coachman - ‎Cited by 1 - ‎Related articles
May 13, 2002 - If a small amount of protein is to be precipitated (less than a few micrograms), add Insulin as a carrier protein (10 micrograms of Sigma insulin ... |
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