[2017-01-11 10:54:52] Beginning TopHat run (v2.1.1)
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[2017-01-11 10:54:52] Checking for Bowtie
Bowtie version: 2.2.9.0
[2017-01-11 10:54:53] Checking for Bowtie index files (genome)..
[2017-01-11 10:54:53] Checking for reference FASTA file
[2017-01-11 10:54:53] Generating SAM header for /Users/SK/my_rna_seq/Homo_sapiens/UCSC/hg19/Sequence/Bowtie2Index/genome
[2017-01-11 10:54:53] Reading known junctions from GTF file
[2017-01-11 10:55:04] Preparing reads
left reads: min. length=101, max. length=101, 27273582 kept reads (108679 discarded)
right reads: min. length=101, max. length=101, 27273582 kept reads (108679 discarded)
[2017-01-11 11:26:08] Building transcriptome data files A549_thout/tmp/genes
[2017-01-11 11:27:08] Building Bowtie index from genes.fa
[2017-01-11 11:41:34] Mapping left_kept_reads to transcriptome genes with Bowtie2
[2017-01-11 14:09:27] Mapping right_kept_reads to transcriptome genes with Bowtie2
[2017-01-11 16:37:02] Resuming TopHat pipeline with unmapped reads
[2017-01-11 16:37:02] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2
[FAILED]
Error running bowtie:
Out of memory allocating plen[] in Ebwt::read() at bt2_io.cpp:272
Error: Encountered exception: 'std::bad_alloc'
Command: /usr/local/bin/../Cellar/bowtie2/2.2.9/bin/bowtie2-align-l --wrapper basic-0 -k 20 -D 15 -R 2 -N 0 -L 20 -i S,1,1.25 --gbar 4 --mp 6,2 --np 1 --rdg 5,3 --rfg 5,3 --score-min C,-14,0 -p 1 --sam-no-hd -x /Users/SK/my_rna_seq/Homo_sapiens/UCSC/hg19/Sequence/Bowtie2Index/genome -
(ERR): bowtie2-align exited with value 1 |
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