Hi, there,
My molecule of interest seems to be localized at TGN.
But our lab does not have antibodies to stain TGN.
So I am wondering if I can use GM130 instead of TGN46 for IF in cultured cells.
Based on publications, GM130 is used to stain cis-Golgi.
TGN46 is for TGN.
My purpose is to co-stain my molecule with TGN marker.
The scope to be used is confocal scope and maximum mag will be x40.
If mag is not quite high such as x100, is it expected to see no staining difference between cis-Golgi and TGN? If so, I want to try...
Any comments and suggestions are welcome.
Sincerely,
XOXO |
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