おお様、
h1もu6もキャップがつくんですね。勘違いをしておりました。
ただいくつかの論文に以下のような記述が見受けられます。
Biochimie
RNAi in mice:
a promising approach to decipher gene functions in vivo
Indeed, several laboratories have developed
new plasmids containing polymerase III promoters, like the
ones of the U6 and H1 genes, known to synthesize small
RNA. Those vectors allow synthesis of 50 bp-long singlestrand
RNA that folded in 21–23 bp dsRNA with a small hairpin
in the middle. These shRNAs lack a 5′ cap and a long
polyadenylated tail [31–33]
BBRC
Simple and efficient DNA vector-based RNAi systems
in mammalian cells
RNA Pol III promoters, especially
H1 and U6 from human and mouse, have been used
most frequently, since they have a well-defined start site
of transcription and a simple effective termination signal
consisting of only five or six consecutive thymidine residues
(Ts), and therefore they are suitable for the synthesis
of small RNA transcripts. Moreover, they can
efficiently transcribe small RNA transcripts lacking both
the 50 cap and 30 polyadenosine [poly(A)] tail [32,33].
ただこれらの引用論文をみてもキャップ構造に関しての記述がございません。
彼らは適当に書いていたのでしょうか。。。 |
|
このスレッドをはてなブックマークに追加