イメージが見れませんでしたけど、もう一つのよく使われる還元剤はTCEP かとおもいます。ほかにも探せば還元剤は見つかりそうですが、、、
泳動中に再酸化という可能性が考えられるなら、以下のようなものもかんがえられます。中身は確か無機硫黄酸化物だったとおもいますが。。。
ちょっと方法論を変えてゲル濾過からむでDTT存在下、非存在下でサイズを見てみてもいいかもしれません、もし再酸化がおこるのであれば。
NuPAGE® Antioxidant
The reducing agents, DTT and ß-mercaptoethanol, do not co-migrate through the gel with the sample in a neutral pH environment of the NuPAGE® Gels. Instead, the reducing agent tends to remain at the top of the gel and not migrate fully throughout the gel. Disulfide bonds are less reactive at neutral pH and are less likely to reoxidize than in a higher pH system. However, in the absence of an antioxidant some reoxidization may occur during the electrophoresis, resulting in slightly diffuse bands.
The NuPAGE® Antioxidant (a proprietary reagent) is added to the running buffer in the upper (cathode) buffer chamber only when performing electrophoresis under reducing conditions. The NuPAGE® Antioxidant migrates with the proteins during electrophoresis preventing the proteins from reoxidizing and maintaining the proteins in a reduced state. The NuPAGE® Antioxidant also protects sensitive amino acids such as methionine and tryptophan from oxidizing.
We also recommend using the NuPAGE® Antioxidant with reduced samples that have been alkylated, for optimal results.
The NuPAGE® Antioxidant is NOT compatible with gel systems other than the NuPAGE® system as the antioxidant is not efficient at higher pHs of other gel systems.
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