論文のMethodに以下のような記述を見つけました。同じ方法を試したいのですが、よくわからない点があります。最後に１％SDSに溶解してタンパク定量とあるのですが、この１％SDSとは単にSDSを水に溶かしたものなのでしょうか。WBにあまり詳しくないので、詳しい方に教えていただければ幸いです。
After treatment, IF-enriched cytoskeletal fractions were obtained
from striatum. Briefly, after the labeling reaction, slices were
homogenized in 400 ml of ice-cold high-salt buffer containing 5 mM
KH2PO4 (pH 7.1), 600 mM KCl, 10 mM MgCl2, 2 mM EGTA, 1 mM
EDTA, 1% Triton X-100 and the protease inhibitors described above.
The homogenate was centrifuged at 15.800×g for 10 min at 4 °C in an
Eppendorf centrifuge, the supernatant discarded and the pellet
homogenized with the same volume of the high-salt medium. The
resuspended homogenate was centrifuged as described and the
supernatant was discarded. The Triton-insoluble IF-enriched pellet,
containing the IFs was dissolved in 1% SDS and protein concentration
was determined.
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