Novogrodsky A, Tal M, Traub A, Hurwitz J.
The enzymatic phosphorylation of ribonucleic acid and
deoxyribonucleic acid. II. Further properties of the 5'-hydroxyl
polynucleotide kinase.
J. Biol. Chem. 241 (1966) 2933-43.
上記論文からの抜粋です。
A、U、G、CTPで同じような活性がみられたとしています。
またgamma-32PATPからの32Pの核酸への取り込みはコールドのATPだけでなくdATPでも見られたことからdATPも燐酸のドナーになる可能性をしてきしています。
The phosphorylating agent was not limited to ATP since GTP,
UTP, and CTP were equally effective as phosphate donors with
DNA as the acceptor (Table III). Similar results were obtained
with RNA as the phosphate acceptor. The K, (millimicromoles
per ml) and Vmax (micromicromoles per 15 min) with each of the
nucleoside triphosphates were: ATP, 14.3 and 40 (see Fig. 3);
UTP, 15 and 33.3; GTP, 33.3 and 66; CTP, 25 and 52, respectively.
At all of the stages of purification, addition of unlabeled
ribonucleoside triphosphates inhibited the phosphorylation of
any of the phosphate acceptors by any labeled nucleoside triphosphates.
This inhibition was also observed with the deoxynucleoside
triphosphates. p,+P-dATP was as effective as
ATP in the phosphorylation of DNA. However, 32P inorganic
polyphosphate was inactive as a phosphate donor. These results
indicate that a variety of different nucleoside triphosphates are
capable of replacing ATP in the kinase reaction.
どんな実験を考えているか分かりませんが、、なるべくATPでやるようにするのがいいとおもいます。というかあまり変える勇気はありません。もし必要でそのような実験をするなら、系が働くが一度見てからのほうがいいかもしれませんね。 |
|
このスレッドをはてなブックマークに追加