I think it depends.
I usually make DTT stock solution in 10 mM sodium acetate to reduce decay and make it last longer.
If your DTT stock solution is made with such acidic buffer and you add substantial volume of the stock in your samples, it is easy to understand pH of the samples are affected.
In this case, the pH would be controled by putting more tris in the sample or make very high concentration of DTT stock solution and minimize the volume of the stock to put in the samples.
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